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Datasheets/Manuals | Printable datasheet for anti-ERBB2 antibody OAEC00074 |
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Predicted Species Reactivity | Human, Mouse, Rat |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | WB, IHC, IF |
Reconstitution and Storage | Store at -20C/1 year |
Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human HER2 around the phosphorylation site of tyrosine 877 (T-E-YP-H-A). |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. |
Concentration | 1 mg/ml |
Specificity | HER2 (phospho-Tyr877) antibody detects endogenous levels of HER2 only when phosphorylated at tyrosine 877. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Application Info | WB: 1:500-1:1000, IHC: 1:50-1:100, IF:1:100-1:200 |
Reference | Dittadi, R. et al. (2000) J. Natl. Cancer Inst. 92, 1443-1444. Muthuswamy, S. K. et al. (1999) Mol. Cell. Biol. 19, 6845-6857. Qian, X. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 1500-1504. |
Gene Symbol | ERBB2 |
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Gene Full Name | Receptor tyrosine-protein kinase erbB-2 |
Alias Symbols | NEU, NGL, HER2, TKR1, CD340, HER-2, MLN 19, HER-2/neu |
NCBI Gene Id | 2064 |
Protein Name | Receptor tyrosine-protein kinase erbB-2 |
Uniprot ID | P04626 |
Protein Accession # | NP_001005862.1 |
Molecular Weight | 138 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "HER2 Antibody (Phospho-Tyr877) (OAEC00074)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat".
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How long will it take to receive "HER2 Antibody (Phospho-Tyr877) (OAEC00074)"?
This item is available "Domestic: within 1 week delivery | International: 1 week".
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What buffer format is "HER2 Antibody (Phospho-Tyr877) (OAEC00074)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "HER2 Antibody (Phospho-Tyr877) (OAEC00074)"?
This target may also be called "NEU, NGL, HER2, TKR1, CD340, HER-2, MLN 19, HER-2/neu" in publications.
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What is the shipping cost for "HER2 Antibody (Phospho-Tyr877) (OAEC00074)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "HER2 Antibody (Phospho-Tyr877) (OAEC00074)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "HER2 Antibody (Phospho-Tyr877) (OAEC00074)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "138 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "HER2 Antibody (Phospho-Tyr877) (OAEC00074)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "ERBB2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "ERBB2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "ERBB2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "ERBB2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "ERBB2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "ERBB2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.