Catalog No: OKBA00029
Size:96 Wells
Price: $1,539.00
SKU
OKBA00029
Availability: Discontinued
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Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Human |
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Application | ELISA |
ELISA Kit Detection Method | Colorimetric, OD450 nm |
ELISA Kit Duration | ~ 3 hours |
ELISA Kit Range | 1 - 81 ng/mL |
ELISA Kit Component | 1. Microtiter wells, 12 x 8 (removable) strips, 96 wells; Wells coated with anti-Hepcidin-25 antibody (monoclonal). 2. Standard, 6 vials, lyophilized, 0.5 mL; Concentrations: 0 - 1 - 3 - 9 - 27 - 81 ng/mL. 3. Control Low & High, 2 vials, lyophilized, 0.5 mL; For control values and ranges please refer to vial label. 4. Sample Diluent, 1 vial, 3 mL, ready to use; Contains non-mercury preservative. 5. Enzyme Conjugate, 1 vial, 7 mL, ready to use, Hepcidin-25 conjugated to biotin; Contains non-mercury preservative. 6. Enzyme Complex, 1 vial, 14 mL, ready to use, Streptavidin conjugated to horseradish peroxidase Contains non-mercury preservative. 7. Substrate Solution, 1 vial, 14 mL, ready to use, Tetramethylbenzidine (TMB). |
Reconstitution and Storage | Store as indicated in product manual. |
Sample Type | Serum, plasma |
Sensitivity | 0.153 ng/mL |
Gene Symbol | HAMP |
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Alias Symbols | HEPC, PLTR, HFE2B, LEAP1 |
NCBI Gene Id | 57817 |
Protein Name | Hepcidin-25 |
Description of Target | The protein encoded by this gene is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids (Hepcidin-25), and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity against bacteria and fungi. Mutations in this gene cause hemochromatosis type 2B, also known as juvenile hemochromatosis, a disease caused by severe iron overload that results in cardiomyopathy, cirrhosis, and endocrine failure. |
Uniprot ID | P81172 |
Protein Accession # | NP_066998.1 |
Nucleotide Accession # | NM_021175.3 |
Protein Size (# AA) | 25 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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