Catalog No: OKCD09125
Size:96WELLS
Price: $800.00
SKU
OKCD09125
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Datasheets/ManualsPrintable datasheet for HEMGN ELISA Kit (Human) (OKCD09125)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hemogen (HEMGN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hemogen (HEMGN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hemogen (HEMGN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Hemogen (HEMGN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hemogen (HEMGN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hemogen (HEMGN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
HEMGN Microplate96 Wells (12 x 8 Well strips)
HEMGN Lyophilized Standard2
100X HEMGN HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.052ng/mL
Gene SymbolHEMGN
Gene Full Namehemogen
Alias SymbolsCT155;EDAG;EDAG-1;erythroid differentiation-associated gene protein;hemogen;hemopoietic gene protein;NDR;negative differentiation regulator protein.
NCBI Gene Id55363
Protein NameHemogen
Description of TargetRegulates the proliferation and differentiation of hematopoietic cells. Overexpression block the TPA-induced megakaryocytic differentiation in the K562 cell model. May also prevent cell apoptosis through the activation of the nuclear factor-kappa B (NF-kB).
Uniprot IDQ9BXL5
Protein Accession #NP_060907.2
Nucleotide Accession #NM_018437.4
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