Catalog No: OCOA03314
Size:20UG
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Datasheets/Manuals | Printable datasheet for HECTD2 Protein Lysate (OCOA03314) |
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Predicted Species Reactivity | Homo sapiens |
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Product Format | Lyophilized |
Host | HEK293T |
Additional Information | Components: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
:: | Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
Reconstitution and Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes |
Tag | C-MYC/DDK |
Description | HECTD2 HEK293T cell transient overexpression lysate (as WB positive control) |
Gene Symbol | HECTD2 |
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Gene Full Name | HECT domain E3 ubiquitin protein ligase 2 |
Alias Symbols | HECT domain containing E3 ubiquitin protein ligase 2;HECT domain-containing protein 2;HECT-type E3 ubiquitin transferase HECTD2;probable E3 ubiquitin-protein ligase HECTD2. |
NCBI Gene Id | 143279 |
Protein Name | B3KV18, NM_182765, NP_877497, Q5U5R9 |
Description of Target | cDNA FLJ16050 fis, clone HHDPC2003820, highly similar to Probable E3 ubiquitin-protein ligase HECTD2, Homo sapiens HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant 1, mRNA, Probable E3 ubiquitin-protein ligase HECTD2, probable E3 ubiquitin-protein ligase HECTD2 isoform a [Homo sapiens] |
Uniprot ID | https://www.uniprot.org/uniprot/Q5U5R9 |
Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/NP_877497 |
Nucleotide Accession # | https://www.ncbi.nlm.nih.gov/nuccore/NM_182765 |
Molecular Weight | 88.1 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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