HDLBP ELISA Kit (Human) (OKCD08172)

Catalog No: OKCD08172

Size:96WELLS

HDLBP ELISA Kit (Human) (OKCD08172) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Density Lipoprotein Binding Protein (HDLBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Density Lipoprotein Binding Protein (HDLBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Density Lipoprotein Binding Protein (HDLBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Density Lipoprotein Binding Protein (HDLBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Density Lipoprotein Binding Protein (HDLBP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Density Lipoprotein Binding Protein (HDLBP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • HDLBP Microplate: 96 Wells (12 x 8 Well strips)
  • HDLBP Lyophilized Standard: 2
  • 100X HDLBP HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.138ng/mL

Target Info

• Gene Symbol: HDLBP
• Gene Full Name: high density lipoprotein binding protein
• Alias Symbols: HBP;HDL-binding protein;High density lipoprotein-binding protein;PRO2900;VGL;vigilin.
• NCBI Gene Id: 3069
• Protein Name: Vigilin
• Description of Target: Appears to play a role in cell sterol metabolism. It may function to protect cells from over-accumulation of cholesterol.
• Uniprot ID: Q00341
• Protein Accession #: NP_001230829.1
• Nucleotide Accession #: NM_001243900.2