HDAC9 ELISA Kit (Human) (OKEH07728)

Catalog No: OKEH07728

Size:96T

Human HDAC9 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	93-103% (n = 20)	102-112% (n = 20)	93-102% (n = 20)	105-115% (n = 20)
  EDTA plasma(n=5)	111-120% (n = 20)	88-97% (n = 20)	100-111% (n = 20)	103-114% (n = 20)
  heparin plasma(n=5)	101-110% (n = 20)	94-105% (n = 20)	104-116% (n = 20)	94-94% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology HDAC9 ELISA Kit (Human) (OKEH07728) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HDAC9 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HDAC9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HDAC9 captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=8.0% (n = 20)
  Mean Inter-assay CV%: <=8.8% (n = 20)
• ELISA Kit Component:
  • HDAC9 Microplate: 96 Wells (12 x 8 Well strips)
  • HDAC9 Lyophilized Standard: 2
  • 100X Biotinylated HDAC9 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter.
  Subcellular Location: Nucleus
  Unigene: Hs.196054
  SMR: Q9UKV0
  String: 9606.ENSP00000408617
  KEGG: hsa:9734
  MIM: 606543
  Pfam: PF12203
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.124ng/mL
• Specificity: Natural and recombinant Human Histone deacetylase 9
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: HDAC9
• Gene Full Name: histone deacetylase 9
• Alias Symbols: HD7;HD7b;HD9;HDAC;HDAC7;HDAC7B;HDAC9B;HDAC9FL;HDRP;histone deacetylase 4/5-related protein;histone deacetylase 7B;histone deacetylase 9;Histone deacetylase-related protein;MEF-2 interacting transcription repressor (MITR) protein;MEF2-interacting transcription repressor MITR;MITR.
• NCBI Gene Id: 9734
• Protein Name: Q9UKV0
• Description of Target: Histone deacetylase 9
• Uniprot ID: https://www.uniprot.org/uniprot/Q9UKV0
• Protein Accession #: NP_001191073.1
• Nucleotide Accession #: NM_001204144.2