HDAC4 (Human) (OKRC01592)

Catalog No: OKRC01592

Size:96 Wells

293 cells were treated with EGF. Cell lysates were treated or untreated with Lambda Protein Phosphatase (LPP) and analyzed using Western Blot.

293 cells were treated with EGF. Cell lysates were treated or untreated with Lambda Protein Phosphatase (LPP) and analyzed using this phospho ELISA.

Jurkat cells were treated with PV (Pervanadate). Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA. Please see step 3 of Part VI Reagent Preparation for detail.

Product Info

• Tested Species Reactivity: Human, Mouse, Rat
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Component:
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Anti-Phospho Antibody
  • HRP-Conjugated Secondary Antibody
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
• Additional Information: Pathway: Epigenetics/chromatin remodeling
  Other materials required:
  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
• Reconstitution and Storage: Upon receipt, the kit should be stored at -20C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge, and store at -20C. Item D, store at 2-8C for up to one month (store at -20C for up to 6 months, avoid repeated freeze-thaw cycles). Reconstituted Positive Control (Item K) should be stored at -70C.
• Sample Type: Cell Lysates, Tissue Lysates
• Specificity: The antibody pair provided in this kit recognizes human/mouse/rat HDAC4 phosphorylated at site Serine-632.
• Assay Info: Assay Methodology: Semi-Quantitative Sandwich ELISA
• Target Post-Translational Modification: Phospho-Ser632
• Introduction: Aviva Systems Biology Phospho-HDAC4 (Ser632) (Histone deacetylase 4) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human, mouse or rat cell lysates. By determining phosphorylated HDAC4 (Histone deacetylase 4) protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
  
  This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human, mouse or rat phospho-HDAC4. An anti-pan HDAC4 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and HDAC4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-HDAC4 (Ser632) antibody is used to detect phosphorylated HDAC4. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HDAC4 (Ser632) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Target Info

• Gene Symbol: HDAC4
• Alias Symbols: Ass, ASSA
• NCBI Gene Id: 25698
• Uniprot ID: P56524