HDAC3 ELISA Kit (Rat) (OKWB00396)

Catalog No: OKWB00396

Size:96T

Rat HDAC3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	87-100%	90-105%	85-99%	90-101%
  EDTA Plasma (n=5)	89-100%	82-97%	82-95%	82-98%
  Heparin Plasma (n=5)	86-99%	81-99%	83-98%	82-99%

• ELISA Kit Principle: Aviva Systems Biology Hdac3 ELISA Kit (Rat) (OKWB00396) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hdac3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hdac3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hdac3 captured in the well.
• ELISA Kit Range: 0.156-10ng/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	91-102	97
  EDTA Plasma (n=5)	85-101	94
  Heparin Plasma (n=5)	85-105	95

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • Hdac3 Microplate: 96 Wells (12 x 8 Well strips)
  • Hdac3 Lyophilized Standard: 2 x 10 ng
  • 100X Hdac3 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 0.094 ng/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Hdac3
• Gene Full Name: histone deacetylase 3
• Alias Symbols: HD3;histone deacetylase 3.
• NCBI Gene Id: 84578
• Protein Name: Histone deacetylase 3
• Description of Target: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation. Contributes, together with XBP1, to the activation of NFE2L2-mediated HMOX1 transcription factor gene expression in a PI(3)K/mTORC2/Akt-dependent signaling pathway leading to endothelial cell (EC) survival under disturbed flow/oxidative stress (By similarity).
• Uniprot ID: Q6P6W3
• Protein Accession #: NP_445900
• Nucleotide Accession #: NM_053448