HDAC2 Antibody - middle region (OAAB06362)

Catalog No: OAAB06362

Size:400ul

HDAC2 Antibody (Center) (Cat. #OAAB06362) western blot analysis in SH-SY5Y cell line lysates (35ug/lane).This demonstrates the HDAC2 antibody detected the HDAC2 protein (arrow).

HDAC2 Antibody (Center) (Cat. #OAAB06362) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HDAC2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

HDAC2 Antibody (Center) (Cat. #OAAB06362) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC-P, FC, IF
• Additional Information: Function: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation. Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A.
  Cellular Location: Nucleus. Cytoplasm
  Tissue Location: Widely expressed; lower levels in brain and lung.
  
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This HDAC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 410-439 amino acids from the Central region of human HDAC2.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Human, Chicken, Mouse
• Intended Use: HDAC2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
  
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
  IF: 1:10~50

Target Info

• Gene Symbol: HDAC2
• Alias Symbols: HD2, RPD3, YAF1, KDAC2
• NCBI Gene Id: 3066
• Protein Name: Histone deacetylase 2
• Description of Target: This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events.
• Uniprot ID: Q92769
• Protein Accession #: NP_001518.3
• Molecular Weight: 55364 Da