HDAC2 Antibody - C-terminal region (OABB00975)

Catalog No: OABB00975 (Formerly GWB-BBP231)

Size:100ug

Anti-HDAC2 antibody, OABB00975, Western blotting
All lanes: Anti HDAC2 (OABB00975) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Predicted bind size: 55 kDa
Observed bind size: 55 kDa

Product Info

• Tested Species Reactivity: Human, Rat
• Predicted Species Reactivity: Mouse
• Product Format: Lyophilized
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: WB, IHC-P
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: Histone deacetylase 2 is an enzyme that in humans is encoded by the HDAC2 gene. This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones(H2A, H2B, H3 and H4). This protein also forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Betz et al.(1998) performed PCR using HDAC2-specific primers to screen a somatic cell hybrid mapping panel. They mapped the HDAC2 gene to human chromosome 6q21, a region of the genome altered in some cancers, including retinoblastoma.
• Reconstitution and Storage: Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.. At -20C for one year from date of receipt. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for six months. Avoid repeated freezing and thawing.
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HDAC2(462-478aa KEEDKSKDNSGEKTDTK), identical to the related rat sequence, and different from the related mouse sequence by one amino acid.
• Purification: Immunogen affinity purified.
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human: Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Rat: Mouse: By Heat
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Histone deacetylase 2(HDAC2) detection. Tested with WB, IHC-P in Human;Rat.

Target Info

• Gene Symbol: HDAC2
• Gene Full Name: histone deacetylase 2
• Alias Symbols: D10Wsu179e, HD 2, HD2, HDAC 2, Hdac2, HDAC2_HUMAN, Histone deacetylase 2(HD2), histone deacetylase 2, OTTHUMP00000017046, OTTHUMP00000227077, OTTHUMP00000227078, RPD3, transcriptional regulator homolog RPD3, YAF1, YY1 associated factor 1, YY1 transcription factor binding protein, Yy1bp
• Protein Name: Histone deacetylase 2(HD2)
• Description of Target: Histone deacetylase 2 is an enzyme that in humans is encoded by the HDAC2 gene. This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones(H2A, H2B, H3 and H4). This protein also forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Betz et al.(1998) performed PCR using HDAC2-specific primers to screen a somatic cell hybrid mapping panel. They mapped the HDAC2 gene to human chromosome 6q21, a region of the genome altered in some cancers, including retinoblastoma.
• Uniprot ID: Q92769