HDAC1 Antibody (OAAF01824)

Catalog No: OAAF01824 (Formerly GWB-ASD095)

Size:100 ug

Western blot analysis of extracts from NIH-3T3 cells, using HDAC1 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using HDAC1 Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of NIH-3T3 cells, using HDAC1 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human HDAC1.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
• Specificity: HDAC1 antibody detects endogenous levels of total HDAC1 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:1000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:10000
• Reference: Richard L. Cai, J. Biol. Chem., Sep 2000; 275: 27909.
  Takayoshi Matsumura, J. Biol. Chem., Apr 2005; 280: 12123 - 12129.
  Gregory David, J. Biol. Chem., Jun 2002; 277: 23658 - 23663.
  Yuh-Long Chang, PNAS, Aug 2001; 98: 9730.

Target Info

• Gene Symbol: HDAC1
• Gene Full Name: histone deacetylase 1
• Alias Symbols: GON-10;HD1;histone deacetylase 1;KDAC1;reduced potassium dependency, yeast homolog-like 1;RPD3;RPD3L1.
• NCBI Gene Id: 3065
• Protein Name: Histone deacetylase 1
• Description of Target: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. Deacetylates NR1D2 and abrogates the effect of KAT5-mediated relieving of NR1D2 transcription repression activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Involved in CIART-mediated transcriptional repression of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex or CRY1 through histone deacetylation.
• Uniprot ID: Q13547
• Protein Accession #: NP_004955.2
• Molecular Weight: 55 kDa