HDAC1 Antibody - C-terminal region (OAAB07311)

Catalog No: OAAB07311

Size:400ul

The anti-HDAC1 Pab (Cat. #OAAB07311) is used in Western blot to detect HDAC1 in ZR-75-1 cell lysate.

The anti-HDAC1 Pab (Cat.#OAAB07311) was used in Western Blot to detect HDAC1 in HEK293 cells. Knockdown of HDAC1 using siRNA against HDAC1 showed a significant decrease of HDAC1 protein using this anti-HDAC1 Pab in HEK293 cells.

HDAC1 is strongly supported by BioGPS gene expression data to be expressed in HEK293, ZR75-1

Confocal immunofluorescent analysis of HDAC1 Antibody (C-term)(Cat#OAAB07311) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IF
• Additional Information: Function: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST- mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. Component a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development
  Cellular Location: Nucleus.
  Tissue Location: Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This HDAC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 449-482 amino acids from the C-terminal region of human HDAC1.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  HDAC1 is strongly supported by BioGPS gene expression data to be expressed in HEK293, ZR75-1

• Intended Use: HDAC1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:2000
  IF: 1:10~50
  WB: 1:1000

Target Info

• Gene Symbol: HDAC1
• Alias Symbols: HD1, RPD3, KDAC1, GON-10, RPD3L1
• NCBI Gene Id: 3065
• Protein Name: Histone deacetylase 1
• Description of Target: Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. HDAC1 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.
• Uniprot ID: Q13547
• Protein Accession #: NP_004955.2
• Molecular Weight: 55103 Da