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Catalog No: OKEH03529
Size:96T
Price: $625.00
SKU
OKEH03529
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology HCRTR2 ELISA Kit (Human) (OKEH03529) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HCRTR2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for HCRTR2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HCRTR2 captured in well.
ELISA Kit Range0.156-10ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 89.9%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 8.4% (n = 20)
Mean Inter-assay CV%: <= 11.1% (n = 20)
ELISA Kit Component
ComponentAmount
HCRTR2 Microplate96 Wells (12 x 8 Well strips)
HCRTR2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated HCRTR2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.085 ng/mL
SpecificityNatural and recombinant Human Orexin receptor type 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolHCRTR2
Gene Full Namehypocretin receptor 2
Alias Symbolshypocretin (orexin) receptor 2;hypocretin receptor type 2;orexin receptor type 2;orexin type-2 receptor;ORXR2;OX2R;OXR2.
NCBI Gene Id3062
Protein NameO43614
Description of TargetOrexin receptor type 2
Uniprot IDhttps://www.uniprot.org/uniprot/O43614
Protein Accession #NP_001517.2
Nucleotide Accession #NM_001526.3
Protein Size (# AA)444
Molecular Weight51 kDa
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