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Datasheets/Manuals | Printable datasheet for HCFC1 Antibody (OAAF02296) |
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Predicted Species Reactivity | Human|Mouse |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human HCFC1. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | HCFC1 antibody detects endogenous levels of total HCFC1 protein. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | IHC: 1:50~1:100; ELISA: 1:20000 |
Reference | Wilson A.C., Cell 74:115-125(1993). Kristie T.M., J. Biol. Chem. 270:4387-4394(1995). Ross M.T., Nature 434:325-337(2005). |
Gene Symbol | HCFC1 |
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Gene Full Name | host cell factor C1 |
Alias Symbols | C1 factor;CFF;HCF;HCF1;HCF-1;HFC1;host cell factor 1;MRX3;PPP1R89;protein phosphatase 1, regulatory subunit 89;VCAF;VP16 accessory protein;VP16-accessory protein. |
NCBI Gene Id | 3054 |
Protein Name | Host cell factor 1 |
Description of Target | Involved in control of the cell cycle (PubMed:10629049, PubMed:10779346, PubMed:15190068, PubMed:16624878, PubMed:23629655). Also antagonizes transactivation by ZBTB17 and GABP2; represses ZBTB17 activation of the p15(INK4b) promoter and inhibits its ability to recruit p300 (PubMed:10675337, PubMed:12244100). Coactivator for EGR2 and GABP2 (PubMed:12244100, PubMed:14532282). Tethers the chromatin modifying Set1/Ash2 histone H3 'Lys-4' methyltransferase (H3K4me) and Sin3 histone deacetylase (HDAC) complexes (involved in the activation and repression of transcription, respectively) together (PubMed:12670868). Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1 (PubMed:20200153). As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues (PubMed:20018852). Recruits KMT2E/MLL5 to E2F1 responsive promoters promoting transcriptional activation and thereby facilitates G1 to S phase transition (PubMed:23629655). |
Uniprot ID | P51610 |
Protein Accession # | NP_005325.2 |
Molecular Weight | 208 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "HCFC1 Antibody (OAAF02296)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse".
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How long will it take to receive "HCFC1 Antibody (OAAF02296)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "HCFC1 Antibody (OAAF02296)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "HCFC1 Antibody (OAAF02296)"?
This target may also be called "C1 factor;CFF;HCF;HCF1;HCF-1;HFC1;host cell factor 1;MRX3;PPP1R89;protein phosphatase 1, regulatory subunit 89;VCAF;VP16 accessory protein;VP16-accessory protein." in publications.
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What is the shipping cost for "HCFC1 Antibody (OAAF02296)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "HCFC1 Antibody (OAAF02296)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "HCFC1 Antibody (OAAF02296)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "208 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "HCFC1 Antibody (OAAF02296)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "HCFC1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "HCFC1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "HCFC1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "HCFC1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "HCFC1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "HCFC1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.