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HBP1 ELISA Kit (Mouse) (OKEH05059)

Catalog#: OKEH05059
Domestic: within 1 week delivery International: 1 week
More Information
Predicted Species Reactivity Mouse
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~3 Hours
Kit Principle Aviva Systems Biology Hbp1 ELISA Kit (Mouse) (OKEH05059) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hbp1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hbp1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hbp1 captured in the well.
Kit Range 0.156 - 10ng/mL
Kit Recovery Mean recovery when spiking into Serum and Plasma = 96.7%
Kit Reproducibility Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <8.5%, n = 20
Mean Inter-Assay Precision - <10.2%, n = 20
Kit Component
HBP1 Microplate96 Wells (12 x 8 Well strips)
HBP1 Lyophilized Standard2
100X Biotinylated HBP1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, plasma and other biological fluids
Sensitivity 0.083 ng/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity Natural and recombinant Mouse HMG box-containing protein 1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol HBP1
Official Gene Full Name High mobility group box transcription factor 1
Alias Symbols 1700058O05Rik, BE629963, C330012F01Rik, C86454, High mobility group box transcription factor 1, HMG box-containing protein 1, HMG box transcription factor 1
NCBI Gene Id 73389
Protein Name HMG box-containing protein 1
Description of Target Transcriptional repressor that binds to the promoter region of target genes. Plays a role in the regulation of the cell cycle and of the Wnt pathway. Binds preferentially to the sequence 5'-TTCATTCATTCA-3'. Binding to the H1F0 promoter is enhanced by interaction with RB1. Disrupts the interaction between DNA and TCF4.
Swissprot Id Q8R316
Protein Accession # NP_694878.2
Nucleotide Accession # NM_153198.2
Protein Size (# AA) 516
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express HBP1.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express HBP1.
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