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Catalog No: OKEH00661
Size:96T
Price: $650.00
SKU
OKEH00661
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH00661
Product Info
Predicted Species ReactivityMouse
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology HbA1c ELISA Kit (Mouse) (OKEH00661) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HbA1c has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HbA1c is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HbA1c captured in well.
ELISA Kit Range3.12-200ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.5% (n = 20)
Mean Inter-assay CV%: < 10.7% (n = 24)
ELISA Kit Component
ComponentAmount
HbA1c Microplate96 Wells (12 x 8 Well strips)
HbA1c Lyophilized Standard2
100X Biotinylated HbA1c Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity1.7 ng/mL
SpecificityNatural and recombinant Mouse Glycosylated hemoglobin A1c
Publications

Amelioration of diabetic nephropathy using pomegranate peel extract-stabilized gold nanoparticles: assessment of NF-κB and Nrf2 signaling system. Int J Nanomedicine. 14, 1753-1777 (2019). 30880978

Mast cell-deficiency protects mice from streptozotocin-induced diabetic cardiomyopathy. Transl Res. 208, 1-14 (2019). 30738862

Osteocalcin is necessary for the alignment of apatite crystallites, but not glucose metabolism, testosterone synthesis, or muscle mass. e1008586 (2020). 32463816

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Gene SymbolHbA1c
Alias Symbolshemoglobin A1c, HbA1c, A1C, or Hb1c
Protein NameGlycated hemoglobin
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