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Catalog No: OKEH00489
Size:96T
Price: $525.00
SKU
OKEH00489
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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HBA1 ELISA Kit (Human) : 96 Wells (OKEH00489)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology HBA1 ELISA Kit (Human) (OKEH00489) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human HBA1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Human HBA1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human HBA1 captured in well.
ELISA Kit Range1.56-100ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.2% (n = 20)
Mean Inter-assay CV%: < 8.7% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-HBA1 Microplate96 Wells (12 x 8 Well strips)
HBA1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated HBA11 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.78 ng/mL
SpecificityNatural and recombinant Human Hemoglobin subunit alpha
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolHBA1, HBA2
Gene Full Namehemoglobin subunit alpha 1, hemoglobin subunit alpha 2
Alias Symbolsalpha globin;alpha globin chain;alpha one globin;alpha-2 globin;alpha-2 globin chain;Alpha-globin;delta globin;ECYT7;HBA-T2;HBA-T3;HBH;hemoglobin alpha 1 globin chain;hemoglobin alpha chain;hemoglobin subunit alpha;hemoglobin, alpha 1;hemoglobin, alpha 2;METHBA;mutant hemoglobin alpha 2 globin chain;truncated HbA2.
NCBI Gene Id3039, 3040
Protein NameP69905
Description of TargetHemoglobin subunit alpha
Uniprot IDhttps://www.uniprot.org/uniprot/P69905
Protein Accession #NP_000508.1
Nucleotide Accession #NM_000517.4
Protein Size (# AA)142
Molecular Weight15 kDa
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