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Havcr1 ELISA Kit (Mouse) : 96 Wells (OKEH02765)

96 Wells
$279.00
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Predicted Species Reactivity:
Mouse
Application:
ELISA
Kit Detection Method:
Colorimetric, OD450 nm
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology Havcr1 ELISA Kit (Mouse) (OKEH02765) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Havcr1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Havcr1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Havcr1 captured in well.
Kit Range:
0.156 - 10ng/mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 107%
Kit Reproducibility:
Mean Intra-assay CV%: < 6.1% (n = 20)
Mean Inter-assay CV%: < 9.5% (n = 20)
Kit Component:
ComponentAmount
Anti-Havcr1 Microplate96 Wells (12 x 8 Well strips)
Havcr1 Lyophilized Standard2
100X Biotinylated Havcr1 Detector Antibody1 x 120 µL
100X Avidin-HRP Conjugate1 x 120 µL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage:
Store as indicated in product manual.
Gene Symbol:
HAVCR1
Official Gene Full Name:
hepatitis A virus cellular receptor 1
Alias Symbols:
AI503787, HAVcr-1, Hepatitis A virus cellular receptor 1 homolog, Kidney injury molecule 1, Kim1, KIM-1, T cell immunoglobulin and mucin domain-containing protein 1, T cell membrane protein 1, Tim1, TIM-1, Timd1, TIMD-1
NCBI Gene Id:
171283
Protein Name:
Hepatitis A virus cellular receptor 1 homolog
Swissprot Id:
Q5QNS5
Protein Accession #:
NP_001160103.1
Nucleotide Accession #:
NM_001166631.1
Sample Type:
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity:
0.088 ng/mL
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express HAVCR1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express HAVCR1.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
Natural and recombinant Mouse Hepatitis A virus cellular receptor 1 homolog
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA
Molecular Weight:
33 kDa
Protein Size (# AA):
305

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