HAVCR1 Chemi-Luminescent ELISA Kit (Mouse) (OKCD03702)

Catalog No: OKCD03702

Size:96WELLS

Mouse HAVCR1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Kidney Injury Molecule 1 (Kim1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Kidney Injury Molecule 1 (Kim1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Kidney Injury Molecule 1 (Kim1) level in the sample or standard.
• ELISA Kit Range: 68.6-50,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kidney Injury Molecule 1 (Kim1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kidney Injury Molecule 1 (Kim1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Havcr1 Microplate: 96 Wells (12 x 8 Well strips)
  • Havcr1 Lyophilized Standard: 2
  • 100X Havcr1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, urine, cell culture supernates and other biological fluids
• Sensitivity: 68.6 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Havcr1
• Gene Full Name: hepatitis A virus cellular receptor 1
• Alias Symbols: AI503787;HAVcr-1;hepatitis A virus cellular receptor 1 homolog;kidney injury molecule 1;KIM-;KIM-1;t cell immunoglobulin and mucin domain-containing protein 1;t cell membrane protein 1;T-cell immunoglobulin and mucin domain containing 1;T-cell immunoglobulin mucin receptor 1;Tim;TIM-;Tim1;TIM-1;Timd1;TIMD-1.
• NCBI Gene Id: 171283
• Protein Name: Hepatitis A virus cellular receptor 1 homolog
• Description of Target: May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4. May play a role in kidney injury and repair (By similarity).
• Uniprot ID: Q5QNS5
• Protein Accession #: NP_001160103
• Nucleotide Accession #: NM_001166631