HAVCR1 Chemi-Luminescent ELISA Kit (Human) (OKCD03701)

Catalog No: OKCD03701

Size:96WELLS

Human HAVCR1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Kidney Injury Molecule 1 (Kim1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Kidney Injury Molecule 1 (Kim1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Kidney Injury Molecule 1 (Kim1) level in the sample or standard.
• ELISA Kit Range: 6.86-5,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kidney Injury Molecule 1 (Kim1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kidney Injury Molecule 1 (Kim1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • HAVCR1 Microplate: 96 Wells (12 x 8 Well strips)
  • HAVCR1 Lyophilized Standard: 2
  • 100X HAVCR1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, urine, cell culture supernates and other biological fluids
• Sensitivity: 0.078 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: HAVCR1
• Gene Full Name: hepatitis A virus cellular receptor 1
• Alias Symbols: CD365;HAVCR;HAVCR-1;hepatitis A virus cellular receptor 1;kidney injury molecule 1;KIM1;KIM-1;T cell immunoglobin domain and mucin domain protein 1;T-cell immunoglobulin and mucin domain-containing protein 1;T-cell immunoglobulin mucin family member 1;T-cell immunoglobulin mucin receptor 1;T-cell membrane protein 1;TIM;TIM1;TIM-1;TIMD1;TIMD-1.
• NCBI Gene Id: 26762
• Protein Name: Hepatitis A virus cellular receptor 1
• Description of Target: May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). May play a role in kidney injury and repair.
• Uniprot ID: Q96D42
• Protein Accession #: NP_001166864
• Nucleotide Accession #: NM_001173393