HAVCR1 Antibody (OABB00585)
|Predicted Species Reactivity||Rat|
|Product Format||LyophilizedEach vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3|
|Application||WB, IHC-P, IHC-F|
|Additional Information||Tissue Specificity: Expressed at a low level in normal kidney but are increased dramatically in postischemic kidney. Expressed in proliferating bromodeoxyuridine-positive and dedifferentiated vimentin-positive epithelial cells in regenerating proximal tubules.
Subcellular Location: Membrane ; Single-pass type I membrane protein.
|Reconstitution and Storage||0.2ml of distilled water will yield a concentration of 500 ug/ml. At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for a longer time. Avoid repeated freezing and thawing|
|Replacement Item||This antibody may replace item sc-135253 from Santa Cruz Biotechnology.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminal of rat KIM1(289-307aa HPRAEDNIYIIEDRSRGAE).|
|Purification||Immunogen affinity purified.|
|Predicted Homology Based on Immunogen Sequence||Rat|
|Datasheets/Manuals||Printable datasheet for anti-HAVCR1 (OABB00585) antibody|
|Specificity||Anti-TIM 1/HAVCR1 Antibody (PA1632) reacts with Rat Havcr1, in native form and recombinant. Superfamily members of Havcr1 are not reactive to PA1632.|
|Application Info||WB: 0.1-0.5 ug/mL. Tested Species: Rat
IHC-P: 0.5-1 ug/mL. Tested: Rat. Antigen retrieval is by heat, where boiling the paraffin sections in 10mM citrate buffer (pH 6.0) for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: 0.5-1 ug/mL. Tested: Rat.
|Target Reference||1. de Souza, A. J., Oriss, T. B., O'Malley, K. J., Ray, A., Kane, L. P. T cell Ig and mucin 1 (TIM-1) is expressed on in vivo-activated T cells and provides a costimulatory signal for T cell activation. Proc. Nat. Acad. Sci. 102: 17113-17118, 2005.
2. Feigelstock, D., Thompson, P., Mattoo, P., Zhang, Y., Kaplan, G. G. The human homolog of HAVcr-1 codes for a hepatitis A virus cellular receptor. J. Virol. 72: 6621-6628, 1998.
Lobb, I; Davison, M; Carter, D; Liu, W; Haig, A; Gunaratnam, L; Sener, A; Hydrogen Sulfide Treatment Mitigates Renal Allograft Ischemia-Reperfusion Injury during Cold Storage and Improves Early Transplant Kidney Function and Survival Following Allogeneic Renal Transplantation. 194, 1806-15 (2015). WB, IHC-P, IHC-F, Rat26239336
|Official Gene Full Name||Hepatitis A virus cellular receptor 1|
|NCBI Gene Id||26762|
|Protein Name||Hepatitis A virus cellular receptor 1|
|Description of Target||KIM1(KIDNEY INJURY MOLECULE 1), also known as HAVCR1, HAVCR or TIM1, is a protein that in humans is encoded by the KIM1 gene. The KIM1 gene is mapped to 5q33.3. Biochemical, mutational, and cell adhesion analyses confirm that Tim1 is capable of homophilic Tim-Tim interactions. The features identified in murine KIM1 is conserved in human KIM1. The KIM1 protein is indeed a receptor for the virus through the infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV. Using a monoclonal antibody to mouse Tim1, Tim1 is expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. Ectopic expression of KIM1 during mouse T-cell differentiation leads to production of the Th2-type cytokine Il4, but not the Th1-type cytokine Ifng. KIM1-expressing epithelial cells internalized apoptotic bodies, and Kim1 is directly responsible for phagocytosis in cultured primary rat tubule epithelial cells and in porcine and canine epithelial cell lines.|
|Protein Accession #||NP_001166864.1; NP_036338.2|
|Nucleotide Accession #||NM_001173393.2; NM_012206.3|
|Molecular Weight||34 kDa|
|Tissue Tool||Find tissues and cell lines supported by DNA array analysis to express HAVCR1.|
|RNA Seq||Find tissues and cell lines supported by RNA-seq analysis to express HAVCR1.|
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol