HAT1 ELISA Kit (Human) (OKEH08239)

Catalog No: OKEH08239

Size:96T

Human HAT1 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology HAT1 ELISA Kit (Human) (OKEH08239) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HAT1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HAT1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HAT1 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=4.9% (n = 20)
  Mean Inter-assay CV%: <=8.8% (n = 20)
• ELISA Kit Component:
  • HAT1 Microplate: 96 Wells (12 x 8 Well strips)
  • HAT1 Lyophilized Standard: 2
  • 100X Biotinylated HAT1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Acetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. May be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. May play a role in DNA repair in response to free radical damage.
  Subcellular Location: Isoform B Cytoplasm Nucleus Nucleus matrix Nucleus Nucleoplasm Localization is predominantly nuclear in normal cells. Treatment with hydrogen peroxide or ionizing radiation enhances nuclear localization through redistribution of existing protein.
  Unigene: Hs.632532
  SMR: O14929
  String: 9606.ENSP00000264108
  KEGG: hsa:8520
  MIM: 603053
  Pfam: PF10394
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.078ng/mL
• Specificity: Natural and recombinant Human Histone acetyltransferase type B catalytic subunit
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: HAT1
• Gene Full Name: histone acetyltransferase 1
• Alias Symbols: Histone acetyltransferase 1;histone acetyltransferase type B catalytic subunit;KAT1.
• NCBI Gene Id: 8520
• Protein Name: O14929
• Description of Target: Histone acetyltransferase type B catalytic subunit
• Uniprot ID: https://www.uniprot.org/uniprot/O14929
• Protein Accession #: NP_003633.1
• Nucleotide Accession #: NM_003642.3