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Catalog No: OKEH00809
Size:96T
Price: $525.00
SKU
OKEH00809
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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HAS2 ELISA Kit (Mouse) : 96 Wells (OKEH00809)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology HAS2 ELISA Kit (Mouse) (OKEH00809) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HAS2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HAS2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HAS2 captured in well.
ELISA Kit Range78-5000pg/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.2% (n = 20)
Mean Inter-assay CV%: < 6.2% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-HAS2 Microplate96 Wells (12 x 8 Well strips)
HAS2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated HAS2 Detector Antibody1 x 120 ul
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity39.3 pg/mL
SpecificityNatural and recombinant Mouse Hyaluronan synthase 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolHas2
Gene Full Namehyaluronan synthase 2
Alias SymbolsHA synthase 2;hyaluronan synthase 2;hyaluronan synthase homolog;hyaluronate synthase 2;hyaluronic acid synthase 2.
NCBI Gene Id15117
Protein NameP70312
Description of TargetHyaluronan synthase 2
Uniprot IDhttps://www.uniprot.org/uniprot/P70312
Protein Accession #NP_032242.3
Nucleotide Accession #NM_008216.3
Protein Size (# AA)552
Molecular Weight63 kDa
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