HAS1 ELISA Kit (Human) (OKCD08170)

Catalog No: OKCD08170

Size:96WELLS

HAS1 ELISA Kit (Human) (OKCD08170) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hyaluronan Synthase 1 (HAS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hyaluronan Synthase 1 (HAS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hyaluronan Synthase 1 (HAS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Hyaluronan Synthase 1 (HAS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hyaluronan Synthase 1 (HAS1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hyaluronan Synthase 1 (HAS1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • HAS1 Microplate: 96 Wells (12 x 8 Well strips)
  • HAS1 Lyophilized Standard: 2
  • 100X HAS1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.053ng/mL

Target Info

• Gene Symbol: HAS1
• Gene Full Name: hyaluronan synthase 1
• Alias Symbols: HA synthase 1;HAS;hyaluronan synthase 1;hyaluronate synthase 1;hyaluronic acid synthase 1.
• NCBI Gene Id: 3036
• Protein Name: Hyaluronan synthase 1
• Description of Target: Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction. Also able to catalyze the synthesis of chito-oligosaccharide depending on the substrate (By similarity).
• Uniprot ID: Q92839
• Protein Accession #: NP_001514.2
• Nucleotide Accession #: NM_001523.3