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Mouse CD30 Antibody : Biotin (OASB01685)

Catalog#: OASB01685
Domestic: within 1 week delivery | International: 1 week
This product is Genway GWB-2F8A44
More Information
Tested Species ReactivityMouse
Additional InformationDescription: CD30 is a type I transmembrane protein and a member of the tumor necrosis factor receptor superfamily of proteins. Murine CD30 is expressed predominantly in the thymus and it is inducible in mouse splenocytes stimulated with pokeweed mitogen or concanavalin A. In anti-CD3 epsilon-activated spleen cells, CD30 is expressed primarily on the surface of CD8+ T cells with peak expression on days 4 and 5. Stimulation of CD30+ CTL lines with plate-bound anti-CD30 directly signals IL-5 but not IFN-gamma production. While these studies demonstrate that CD30 directs cytokine secretion and suggest that CD30 may play a pivotal role in the pattern of cytokine production by T cells, the precise roles of CD30 and its ligand (CD153) in T-cell development have not been clearly defined.
Reconstitution and StorageStore at 2-8C
Replacement ItemThis antibody may replace item sc-19658 from Santa Cruz Biotechnology.
ImmunogenMouse CD30-mouse IgG1 fusion protein
Concentration0.5 mg/mL
Datasheets/ManualsPrintable datasheet for anti-TNFRSF8 (OASB01685) antibody
CharacterizationTo ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conformto characteristics of a standard reference reagent.
WarningReagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact withskin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, washwith copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azideyields toxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running waterbefore discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
DilutionFlow Cytometry: Purified antibody <= 3 ug/106 cells
Fluorescein conjugate <= 3 ug/106 cells
Biotin conjugate <= 3 ug/106 cells
R-phycoerythrin conjugate <= 0.2 ug/106 cells
Application InfoFlow cytometry 1, In vitro functional studies 1
Other Applications DataSince applications vary, you should determine the optimum working dilution for the product that is appropriate for yourspecific need.
Other Applications Image 1 Data Amount Used: 3 Ug/106 cells The YAC-1 cell line was stained with hamster antimouse CD30-FITC. Large cells were gated and analyzed by flow cytometry.
Target Reference1. Bowen, M.A., R.K. Lee, G. Miragliotta, S.Y. Nam, and E.R. Podack. 1996. J. Immunol. 156:442
2. Falini, B., et al. 1995. Blood 85:1
3. Shanebeck, K. D., et al. 1995. Eur. J. Immunol. 25:2147.
Storage- The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0mL of PBS. Aliquot and store at or below - 20 C
- Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected.
Gene SymbolTNFRSF8
Official Gene Full NameTumor necrosis factor receptor superfamily member 8
Alias SymbolsKi, Cd30, Ki-1, D1S166E
NCBI Gene Id21941
Protein Nametumor necrosis factor receptor superfamily member 8
Swissprot IdQ60846
Protein Accession #NP_033427.1
Nucleotide Accession #NM_009401.2
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express TNFRSF8
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express TNFRSF8
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