HAMP ELISA Kit (Rat) (OKEH03433)

Catalog No: OKEH03433

Size:96T

Rat HAMP ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology HAMP ELISA Kit (Rat) (OKEH03433) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Rat Hamp antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Hamp and incubated. The Hamp found in the sample or standards competes with the biotinylated Hamp for limited binding sites on the immobilized anti-Rat Hamp antibody. Excess unbound biotinylated Hamp and sample or standard Hamp is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Hamp captured in the well and inversely proportional to the amount of Hamp which was contained in the sample or standard.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 99%
• ELISA Kit Reproducibility: Mean Intra-Assay Precision: %CV <4.7%, n=20, Mean Inter-Assay Precision: %CV <5.4%, n=20
• ELISA Kit Component:
  • Anti-Hamp Microplate: 96 Wells (12 x 8 Well strips)
  • Hamp Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Hamp-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 39 pg/mL
• Specificity: Natural and recombinant Rat Hepcidin
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: Hamp
• Gene Full Name: hepcidin antimicrobial peptide
• Alias Symbols: Hepc;hepcidin.
• NCBI Gene Id: 84604
• Protein Name: Q99MH3
• Description of Target: Hepcidin
• Uniprot ID: https://www.uniprot.org/uniprot/Q99MH3
• Protein Accession #: NP_445921.1
• Nucleotide Accession #: NM_053469.1
• Protein Size (# AA): 84
• Molecular Weight: 9 kDa