HAMP ELISA Kit (Dog) (OKEH08457)

Catalog No: OKEH08457

Size:96T

Product Info

• Predicted Species Reactivity: Canis lupus, Dog
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology HAMP ELISA Kit (Dog) (OKEH08457) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HAMP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HAMP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HAMP captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=8.3% (n = 20)
  Mean Inter-assay CV%: <=12.2% (n = 20)
• ELISA Kit Component:
  • HAMP Microplate: 96 Wells (12 x 8 Well strips)
  • HAMP Lyophilized Standard: 2
  • 100X Biotinylated HAMP Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages. May also have antimicrobial activity.
  Subcellular Location: Secreted
  Unigene: Cfa.14486
  SMR: Q5U9D2
  String: 9615.ENSCAFP00000010474
  KEGG: cfa:492281
  Pfam: PF06446
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Dog Hepcidin
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: HAMP
• Gene Full Name: hepcidin antimicrobial peptide
• Alias Symbols: hepcidin.
• NCBI Gene Id: 492281
• Protein Name: Q5U9D2
• Description of Target: Hepcidin
• Uniprot ID: https://www.uniprot.org/uniprot/Q5U9D2
• Protein Accession #: NP_001007141.1
• Nucleotide Accession #: NM_001007140.1