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Catalog No: OKCD07863
Size:96WELLS
Price: $825.00
SKU
OKCD07863
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Datasheets/ManualsPrintable datasheet for HAMP ELISA Kit (Bovine) (OKCD07863)
Product Info
Predicted Species ReactivityBos taurus|Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepcidin (Hepc). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hepcidin (Hepc). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepcidin (Hepc), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Hepcidin (Hepc) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range31.2-2,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepcidin (Hepc) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepcidin (Hepc) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
HAMP Microplate96 Wells (12 x 8 Well strips)
HAMP Lyophilized Standard2
100X HAMP HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids.
Sensitivity< 0.214ng/mL
Gene SymbolHAMP
Gene Full Namehepcidin antimicrobial peptide
Alias Symbolshepcidin.
NCBI Gene Id512301
Protein NameHepcidin antimicrobial peptide
Description of TargetHepcidin antimicrobial peptide
Uniprot IDQ2NKT0
Protein Accession #NP_001107980.1
Nucleotide Accession #NM_001114508.2
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