HAMP Chemi-Luminescent ELISA Kit (Human) (OKCD03551)

Catalog No: OKCD03551

Size:96WELLS

Human HAMP ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	84-103%	80-103%	79-102%	85-93%
  EDTA Plasma (n=5)	87-95%	83-104%	86-99%	92-104%
  Heparin Plasma (n=5)	88-102%	78-95%	97-104%	82-96%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Hepcidin (Hepc). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Hepcidin (Hepc). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Hepcidin (Hepc) level in the sample or standard.
• ELISA Kit Range: 5.49-4,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	81-99	93
  EDTA Plasma (n=5)	78-101	91
  Heparin Plasma (n=5)	82-102	90

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepcidin (Hepc) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepcidin (Hepc) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • HAMP Microplate: 96 Wells (12 x 8 Well strips)
  • HAMP Lyophilized Standard: 2
  • 100X HAMP Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 62.5 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: HAMP
• Gene Full Name: hepcidin antimicrobial peptide
• Alias Symbols: HEPC;hepcidin;hepcidin preproprotein;HFE2B;LEAP1;liver-expressed antimicrobial peptide 1;PLTR;putative liver tumor regressor.
• NCBI Gene Id: 57817
• Protein Name: Hepcidin
• Description of Target: Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes (PubMed:22306005).
• Uniprot ID: P81172
• Protein Accession #: NP_066998
• Nucleotide Accession #: NM_021175