Catalog No: OKCD00714 (Formerly GWB-KBBCX1)
Size:96WELLS
Price: $700.00
SKU
OKCD00714
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Datasheets/ManualsPrintable datasheet for HADHB ELISA Kit (Mouse) (OKCD00714)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
98-105%92-99%95-103%80-91%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hydroxyacyl Coenzyme A Dehydrogenase Beta (HADHb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hydroxyacyl Coenzyme A Dehydrogenase Beta (HADHb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hydroxyacyl Coenzyme A Dehydrogenase Beta (HADHb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Hydroxyacyl Coenzyme A Dehydrogenase Beta (HADHb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.56-100ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
98-105101
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hydroxyacyl Coenzyme A Dehydrogenase Beta (HADHb) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hydroxyacyl Coenzyme A Dehydrogenase Beta (HADHb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Hadhb Microplate96 Wells (12 x 8 Well Strips)
Hadhb Lyophilized Standard2 x 100 ng
100X Biotinylated Hadhb Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids.
Sensitivity< 0.59 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Hydroxyacyl Coenzyme A Dehydrogenase Beta (HADHb). No significant cross-reactivity or interference between Hydroxyacyl Coenzyme A Dehydrogenase Beta (HADHb) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolHadhb
Gene Full Namehydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta
Alias Symbols4930479F15Rik;hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta aubunit;hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta subunit;Mtp;Mtpb;TP-beta;trifunctional enzyme subunit beta, mitochondrial.
NCBI Gene Id231086
Protein NameTrifunctional enzyme subunit beta, mitochondrial
Description of TargetTrifunctional enzyme subunit beta, mitochondrial
Uniprot IDQ99JY0
Protein Accession #NP_001276727
Nucleotide Accession #NM_001289798.1
Protein Size (# AA)475
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