HADHB ELISA Kit (Human) (OKEH08010)

Catalog No: OKEH08010

Size:96T

Human HADHB ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	86-97% (n = 20)	99-99% (n = 20)	106-115% (n = 20)	105-114% (n = 20)
  EDTA plasma(n=5)	89-98% (n = 20)	105-116% (n = 20)	100-112% (n = 20)	97-106% (n = 20)
  heparin plasma(n=5)	89-98% (n = 20)	101-111% (n = 20)	106-114% (n = 20)	113-123% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology HADHB ELISA Kit (Human) (OKEH08010) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HADHB has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HADHB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HADHB captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=8.3% (n = 20)
  Mean Inter-assay CV%: <=9.3% (n = 20)
• ELISA Kit Component:
  • HADHB Microplate: 96 Wells (12 x 8 Well strips)
  • HADHB Lyophilized Standard: 2
  • 100X Biotinylated HADHB Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
  
  Subcellular Location: Mitochondrion Mitochondrion inner membrane Mitochondrion outer membrane Endoplasmic reticulum
  Unigene: Hs.515848
  String: 9606.ENSP00000325136
  KEGG: hsa:3032
  MIM: 143450
  Pfam: PF02803
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.048ng/mL
• Specificity: Natural and recombinant Human Trifunctional enzyme subunit beta, mitochondrial
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: HADHB
• Gene Full Name: hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta
• Alias Symbols: 2-enoyl-Coenzyme A (CoA) hydratase, beta subunit;3-ketoacyl-Coenzyme A (CoA) thiolase of mitochondrial trifunctional protein, beta subunit;acetyl-CoA acyltransferase;beta-ketothiolase;ECHB;hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), beta subunit;hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta subunit;MSTP029;MTPB;TP-BETA;trifunctional enzyme subunit beta, mitochondrial.
• NCBI Gene Id: 3032
• Protein Name: P55084
• Description of Target: Trifunctional enzyme subunit beta, mitochondrial
• Uniprot ID: https://www.uniprot.org/uniprot/P55084
• Protein Accession #: NP_000174.1
• Nucleotide Accession #: NM_000183.2