Catalog No: OKEH08009
Size:96T
Price: $725.00
SKU
OKEH08009
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
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Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | Aviva Systems Biology HADHA ELISA Kit (Human) (OKEH08009) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HADHA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HADHA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HADHA captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 0.625-40ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 117% (n = 20) | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: <=5.4% (n = 20) Mean Inter-assay CV%: <=9.0% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | Function: Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway (PubMed:8135828, PubMed:1550553, PubMed:29915090, PubMed:30850536). The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA (PubMed:29915090). Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids (PubMed:30850536). Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA described here carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities while the trifunctional enzyme subunit beta/HADHB bears the 3-ketoacyl-CoA thiolase activity (PubMed:8135828, PubMed:29915090, PubMed:30850536). Independently of the subunit beta, the trifunctional enzyme subunit alpha/HADHA also has a monolysocardiolipin acyltransferase activity (PubMed:23152787). It acylates monolysocardiolipin into cardiolipin, a major mitochondrial membrane phospholipid which plays a key role in apoptosis and supports mitochondrial respiratory chain complexes in the generation of ATP (PubMed:23152787). Allows the acylation of monolysocardiolipin with different acyl-CoA substrates including oleoyl-CoA for which it displays the highest activity (PubMed:23152787). | ||||||||||||||||||||||
:: | Subcellular Location: Mitochondrion Mitochondrion inner membrane Protein stability and association with mitochondrion inner membrane do not require HADHB. | ||||||||||||||||||||||
:: | Unigene: Hs.516032 SMR: P40939 String: 9606.ENSP00000370023 | ||||||||||||||||||||||
:: | KEGG: hsa:3030 MIM: 600890 Pfam: PF00725 | ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sensitivity | 0.302ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human Trifunctional enzyme subunit alpha, mitochondrial | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | HADHA |
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Gene Full Name | hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha |
Alias Symbols | 3-ketoacyl-Coenzyme A (CoA) thiolase, alpha subunit;3-oxoacyl-CoA thiolase;78 kDa gastrin-binding protein;ECHA;gastrin-binding protein;GBP;HADH;hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), alpha subunit;hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit;LCEH;LCHAD;long-chain 2-enoyl-CoA hydratase;long-chain-3-hydroxyacyl-CoA dehydrogenase;mitochondrial long-chain 2-enoyl-Coenzyme A (CoA) hydratase, alpha subunit;mitochondrial long-chain L-3-hydroxyacyl-Coenzyme A (CoA) dehydrogenase, alpha subunit;mitochondrial trifunctional enzyme, alpha subunit;mitochondrial trifunctional protein, alpha subunit;monolysocardiolipin acyltransferase;MTPA;TP-ALPHA;trifunctional enzyme subunit alpha, mitochondrial. |
NCBI Gene Id | 3030 |
Protein Name | P40939 |
Description of Target | Trifunctional enzyme subunit alpha, mitochondrial |
Uniprot ID | https://www.uniprot.org/uniprot/P40939 |
Protein Accession # | NP_000173.2 |
Nucleotide Accession # | NM_000182.4 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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