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Catalog No: OKEH08009
Size:96T
Price: $725.00
SKU
OKEH08009
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Linearity
Sample1:21:41:81:16
serum(n=5)105-114% (n = 20)80-90% (n = 20)109-118% (n = 20)87-99% (n = 20)
EDTA plasma(n=5)94-104% (n = 20)91-101% (n = 20)98-107% (n = 20)87-100% (n = 20)
heparin plasma(n=5)81-91% (n = 20)87-97% (n = 20)93-103% (n = 20)80-90% (n = 20)
ELISA Kit PrincipleAviva Systems Biology HADHA ELISA Kit (Human) (OKEH08009) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HADHA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HADHA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HADHA captured in well.
ELISA Kit Range0.625-40ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 117% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=5.4% (n = 20)
Mean Inter-assay CV%: <=9.0% (n = 20)
ELISA Kit Component
ComponentAmount
HADHA Microplate96 Wells (12 x 8 Well strips)
HADHA Lyophilized Standard2
100X Biotinylated HADHA Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway (PubMed:8135828, PubMed:1550553, PubMed:29915090, PubMed:30850536). The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA (PubMed:29915090). Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids (PubMed:30850536). Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA described here carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities while the trifunctional enzyme subunit beta/HADHB bears the 3-ketoacyl-CoA thiolase activity (PubMed:8135828, PubMed:29915090, PubMed:30850536). Independently of the subunit beta, the trifunctional enzyme subunit alpha/HADHA also has a monolysocardiolipin acyltransferase activity (PubMed:23152787). It acylates monolysocardiolipin into cardiolipin, a major mitochondrial membrane phospholipid which plays a key role in apoptosis and supports mitochondrial respiratory chain complexes in the generation of ATP (PubMed:23152787). Allows the acylation of monolysocardiolipin with different acyl-CoA substrates including oleoyl-CoA for which it displays the highest activity (PubMed:23152787).
::Subcellular Location: Mitochondrion Mitochondrion inner membrane Protein stability and association with mitochondrion inner membrane do not require HADHB.
::Unigene: Hs.516032
SMR: P40939
String: 9606.ENSP00000370023
::KEGG: hsa:3030
MIM: 600890
Pfam: PF00725
Reconstitution and StorageStore as indicated in product manual.
Sensitivity0.302ng/mL
SpecificityNatural and recombinant Human Trifunctional enzyme subunit alpha, mitochondrial
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolHADHA
Gene Full Namehydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha
Alias Symbols3-ketoacyl-Coenzyme A (CoA) thiolase, alpha subunit;3-oxoacyl-CoA thiolase;78 kDa gastrin-binding protein;ECHA;gastrin-binding protein;GBP;HADH;hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), alpha subunit;hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit;LCEH;LCHAD;long-chain 2-enoyl-CoA hydratase;long-chain-3-hydroxyacyl-CoA dehydrogenase;mitochondrial long-chain 2-enoyl-Coenzyme A (CoA) hydratase, alpha subunit;mitochondrial long-chain L-3-hydroxyacyl-Coenzyme A (CoA) dehydrogenase, alpha subunit;mitochondrial trifunctional enzyme, alpha subunit;mitochondrial trifunctional protein, alpha subunit;monolysocardiolipin acyltransferase;MTPA;TP-ALPHA;trifunctional enzyme subunit alpha, mitochondrial.
NCBI Gene Id3030
Protein NameP40939
Description of TargetTrifunctional enzyme subunit alpha, mitochondrial
Uniprot IDhttps://www.uniprot.org/uniprot/P40939
Protein Accession #NP_000173.2
Nucleotide Accession #NM_000182.4
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