HADH Antibody (OAGA02490)

Catalog No: OAGA02490

Size:100 ul

HADH antibody detects HADH protein by Western blot analysis.
A. 30 ug 293T whole cell lysate/extract
B. 30 ug A431 whole cell lysate/extract
C. 30 ug HeLa whole cell lysate/extract
D. 30 ug HepG2 whole cell lysate/extract
10 % SDS-PAGE
HADH antibody (OAGA02490) dilution: 1:1000

HADH antibody detects HADH protein by Western blot analysis.
A. 30 ug GL261 whole cell lysate/extract
B. 30 ug C8D30 whole cell lysate/extract
10 % SDS-PAGE
HADH antibody (OAGA02490) dilution: 1:1000

HADH antibody detects HADH protein by Western blot analysis.
A. 30 ug PC-12 whole cell lysate/extract
10 % SDS-PAGE
HADH antibody (OAGA02490) dilution: 1:1000

HADH antibody detects HADH protein at mitochondria on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
HADH antibody (OAGA02490) dilution: 1:500.

HADH antibody detects HADH protein at mitochondria on mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
HADH antibody (OAGA02490) dilution: 1:500.

HADH antibody detects HADH protein at Mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37C for 30 min.
Green: HADH protein stained by HADH antibody (OAGA02490) diluted at 1:500.
Blue: Hoechst 33343 staining.

Immunoprecipitation of HADH protein from HepG2 whole cell extracts using 5 ug of HADH antibody (OAGA02490).
Western blot analysis was performed using HADH antibody (OAGA02490).
EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. 1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IF, IHC-P, IP, WB
• Additional Information: Cellular localization: Mitochondrion matrix
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human HADH. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Predicted Homology Based on Immunogen Sequence: Xenopus laevis (82%), Pig (95%), Chicken (80%), Rhesus Monkey (98%), Bovine (91%)
• Concentration: 1mg/ml
• Application Info: Suggested starting dilutions are as follows:
  WB: 1:500-1:3000,
  IHC-P: 1:100-1:1000,
  ICC/IF: 1:100-1:1000,
  IP: 1:100-1:500.
  Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Target Info

• Gene Symbol: HADH
• Gene Full Name: hydroxyacyl-CoA dehydrogenase
• Alias Symbols: HAD, HCDH, HHF4, HADH1, SCHAD, HADHSC, MSCHAD
• NCBI Gene Id: 3033
• Description of Target: This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene of this gene on chromosome 15.
• Uniprot ID: Q16836