HACE1 ELISA Kit (Chicken) (OKEH08598)

Catalog No: OKEH08598

Size:96T

Product Info

• Predicted Species Reactivity: Chicken, Gallus gallus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology HACE1 ELISA Kit (Chicken) (OKEH08598) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HACE1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HACE1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HACE1 captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 115% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=6.8% (n = 20)
  Mean Inter-assay CV%: <=8.3% (n = 20)
• ELISA Kit Component:
  • HACE1 Microplate: 96 Wells (12 x 8 Well strips)
  • HACE1 Lyophilized Standard: 2
  • 100X Biotinylated HACE1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division.
  Subcellular Location: Golgi apparatus Golgi stack membrane Cytoplasm Endoplasmic reticulum
  Unigene: Gga.22685
  String: 9031.ENSGALP00000024820
  KEGG: gga:421788
  Pfam: PF12796
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Chicken E3 ubiquitin-protein ligase HACE1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: HACE1
• Gene Full Name: HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1
• Alias Symbols: E3 ubiquitin-protein ligase HACE1;HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1;HECT-type E3 ubiquitin transferase HACE1.
• NCBI Gene Id: 421788
• Protein Name: E1C656
• Description of Target: E3 ubiquitin-protein ligase HACE1
• Uniprot ID: https://www.uniprot.org/uniprot/E1C656
• Protein Accession #: NP_001265084.1
• Nucleotide Accession #: NM_001278155.1