HACD1 ELISA Kit (Human) (OKCD01257)

Catalog No: OKCD01257 (Formerly GWB-KBBRR6)

Size:96WELLS

Human HACD1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Tyrosine Phosphatase Like Protein A (PTPLA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Tyrosine Phosphatase Like Protein A (PTPLA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Tyrosine Phosphatase Like Protein A (PTPLA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Protein Tyrosine Phosphatase Like Protein A (PTPLA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Tyrosine Phosphatase Like Protein A (PTPLA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Tyrosine Phosphatase Like Protein A (PTPLA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-HACD1 Microplate: 96 Wells (12 x 8 Well Strips)
  • HACD1 Lyophilized Standard: 2 x 50 ng
  • 100X Biotinylated HACD1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.27 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Protein Tyrosine Phosphatase Like Protein A (PTPLA). No significant cross-reactivity or interference between Protein Tyrosine Phosphatase Like Protein A (PTPLA) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: HACD1
• Gene Full Name: 3-hydroxyacyl-CoA dehydratase 1
• Alias Symbols: 3-hydroxyacyl-CoA dehydratase 1;CAP;cementum attachment protein;Cementum-attachment protein;protein tyrosine phosphatase-like (proline instead of catalytic arginine), member A;Protein-tyrosine phosphatase-like member A;PTPLA;very-long-chain (3R)-3-hydroxyacyl-[acyl-carrier protein] dehydratase 1;very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 1.
• NCBI Gene Id: 9200
• Protein Name: Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 1
• Description of Target: Isoform 1: Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.
• Uniprot ID: B0YJ81
• Protein Accession #: NP_055056
• Nucleotide Accession #: NM_014241.3
• Protein Size (# AA): 288