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Catalog No: OKEH07673
Size:96T
Price: $800.00
SKU
OKEH07673
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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HAAO ELISA Kit (Bovine) (OKEH07673)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology HAAO ELISA Kit (Bovine) (OKEH07673) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HAAO has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HAAO is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HAAO captured in well.
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 97% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=7.9% (n = 20)
Mean Inter-assay CV%: <=12.4% (n = 20)
ELISA Kit Component
ComponentAmount
HAAO Microplate96 Wells (12 x 8 Well strips)
HAAO Lyophilized Standard2
100X Biotinylated HAAO Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.
::Subcellular Location: Cytoplasm
::Unigene: Bt.20241
SMR: Q0VCA8
String: 9913.ENSBTAP00000006132
::KEGG: bta:510602
Pfam: PF06052
Reconstitution and StorageStore as indicated in product manual.
SpecificityNatural and recombinant Bovine 3-hydroxyanthranilate 3,4-dioxygenase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolHAAO
Gene Full Name3-hydroxyanthranilate 3,4-dioxygenase
Alias Symbols3-HAO;3-hydroxyanthranilate 3,4-dioxygenase;3-hydroxyanthranilate oxygenase;3-hydroxyanthranilic acid dioxygenase;HAD.
NCBI Gene Id510602
Protein NameQ0VCA8
Description of Target3-hydroxyanthranilate 3,4-dioxygenase
Uniprot IDhttps://www.uniprot.org/uniprot/Q0VCA8
Protein Accession #NP_001068926.1
Nucleotide Accession #NM_001075458.2
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