HA Tag Antibody (OAAB07238)

Catalog No: OAAB07238 (Formerly GWB-3K99AE)

Size:200UL

Western blot analysis of anti-HA tag pab(Cat. #OAAB07238)in HA-tagged recombinant protein bacterial lysate.

ChIP was performed with 35S:HATCL1 plants using anti-HA antibodies. Rabbit preimmune serum was used as a mock control. Primer sets specific for the first intron or the 3-UTR region of GL1 were used in PCR reactions. ACTIN2 provided a control(Provided by Shucai Wang, Su-Hwan Kwak:Development 134, 3873-3882 (2007)

GTPase activity of dynamin-2 is required for endocytosis of cell-surface tTG. MRC-5 fibroblasts were transiently transfected with either wild-type (wt) or a GTPase-deficient dynamin-2 mutant (K44A) with a hemagglutinin (HA) tag.(Provided by Evgeny A. Zemskov, Irina Mikhailenko:Journal of Cell Science 120, 3188-3199 (2007)

Product Info

• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: Chromatin Immunoprecipitation, Immunofluorescence, Western blot
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA) was used as antigen.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: 2 mg/ml
• Intended Use: HA Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  IF: 1:10~50
  CHIP: 1:100

Target Info

• Alias Symbols: Glutathione S-transferase
• Description of Target: Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYAYPYDVPDYA) is an established example. Aviva's anti-HA polyclonal antibody provides a simple solution to detect the expression of an HA-tagged protein in cells.