H6PD ELISA Kit (Mouse) (OKWB00252)

Catalog No: OKWB00252

Size:96T

Mouse H6PD ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	85-104%	90-103%	88-105%	87-103%
  EDTA Plasma (n=5)	88-101%	85-100%	86-101%	82-99%
  Heparin Plasma (n=5)	81-96%	82-95%	82-100%	80-100%

• ELISA Kit Principle: Aviva Systems Biology H6pd ELISA Kit (Mouse) (OKWB00252) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for H6pd has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for H6pd is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample H6pd captured in the well.
• ELISA Kit Range: 0.156-10ng/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	87-96	93
  EDTA Plasma (n=5)	93-101	98
  Heparin Plasma (n=5)	87-103	92

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • H6pd Microplate: 96 Wells (12 x 8 Well strips)
  • H6pd Lyophilized Standard: 2 x 10 U
  • 100X H6pd Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 0.094 mU/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: H6pd
• Gene Full Name: hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase)
• Alias Symbols: AI785303;G6pd;G6pd1;GDH/6PGL endoplasmic bifunctional protein;glucose 1-dehydrogenase;glucose-6-phosphate dehydrogenase 1;Gpd;Gpd-;Gpd1;Gpd-1.
• NCBI Gene Id: 100198
• Protein Name: GDH/6PGL endoplasmic bifunctional protein
• Description of Target: Oxidizes glucose-6-phosphate and glucose, as well as other hexose-6-phosphates.
• Uniprot ID: Q8CFX1
• Protein Accession #: NP_001277933
• Nucleotide Accession #: NM_001291004