H3F3A ELISA Kit (Human) (OKEH01773)

Catalog No: OKEH01773

Size:96T

Human H3F3A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology H3F3A ELISA Kit (Human) (OKEH01773) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for H3F3A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for H3F3A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample H3F3A captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 3.5% (n = 20)
  Mean Inter-assay CV%: < 7.9% (n = 20)
• ELISA Kit Component:
  • H3F3A Microplate: 96 Wells (12 x 8 Well strips)
  • H3F3A Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated H3F3A Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.181 ng/mL
• Specificity: Natural and recombinant Human Histone H3.3
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: H3-3A, H3-3B
• Gene Full Name: H3.3 histone A, H3.3 histone B
• Alias Symbols: H3 histone family member 3A;H3 histone family member 3B;H3 histone, family 3A;H3 histone, family 3B (H3.3B);H3.3A;H3.3B;H3-3A;H3-3B;H3F3;H3F3A;H3F3B;histone H3.3.
• NCBI Gene Id: 3020, 3021
• Protein Name: P84243
• Description of Target: Histone H3.3
• Uniprot ID: https://www.uniprot.org/uniprot/P84243
• Protein Accession #: NP_001013721.2
• Nucleotide Accession #: NM_001013699.2
• Protein Size (# AA): 135
• Molecular Weight: 15 kDa