H3-3B ELISA Kit (Bovine) (OKEH08624)

Catalog No: OKEH08624

Size:96T

Bovine H3-3B ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology H3-3B, H3F3B ELISA Kit (OKEH08624) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-H3-3B, H3F3B antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated H3-3B, H3F3B and incubated. The H3-3B, H3F3B found in the sample or standards competes with the biotinylated H3-3B, H3F3B for limited binding sites on the immobilized anti-H3-3B, H3F3B antibody. Excess unbound biotinylated H3-3B, H3F3B and sample or standard H3-3B, H3F3B is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated H3-3B, H3F3B captured in the well and inversely proportional to the amount of H3-3B, H3F3B which was contained in the sample or standard.
• ELISA Kit Range: 0.625-40ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 85% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=5.5% (n = 20)
  Mean Inter-assay CV%: <=11.6% (n = 20)
• ELISA Kit Component:
  • Anti-H3-3B, H3F3B Microplate: 96 Wells (12 x 8 Well strips)
  • H3-3B, H3F3B Lyophilized Standard: 2 x 100 ng
  • Sample Diluent: 1 x 20 mL
  • 100X H3-3B, H3F3B-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  Subcellular Location: Nucleus Chromosome
  Unigene: Bt.15474
  SMR: Q5E9F8
  String: 9913.ENSBTAP00000034581
  KEGG: bta:326601
  Pfam: PF00125
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.274ng/mL
• Specificity: Natural and recombinant Bovine Histone H3.3
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: H3-3A, H3-3B
• Gene Full Name: H3.3 histone A, H3.3 histone B
• Alias Symbols: H3 histone, family 3A;H3 histone, family 3B;H3.3B;H3-3B;H3F3A;H3F3B;histone H3.3.
• NCBI Gene Id: 326601, 617051
• Protein Name: Q5E9F8
• Description of Target: Histone H3.3
• Uniprot ID: https://www.uniprot.org/uniprot/Q5E9F8
• Protein Accession #: NP_001014411.1
• Nucleotide Accession #: NM_001014389.2