H2AFZ Antibody (OAGA00660)

Catalog No: OAGA00660

Size:100 ul

Sample(30 ug of whole cell lysate)
A:MOLT4
B:Raji
12% SDS PAGE
OAGA00660 diluted at 1:3000

Immunohistochemical analysis of paraffin-embedded ES-2 xenograft, using Histone H2A.Z(OAGA00660) antibody at 1:100 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Histone H2A.Z(OAGA00660) antibody at 1:500 dilution.

Histone H2A.Z antibody immunoprecipitates Histone H2A.Z protein in IP experiments. IP Sample: 1000 ug HeLa whole cell lysate/extract A. 50 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of Histone H2A.Z protein by 2 ug of Histone H2A.Z antibody (OAGA00660) 15% SDS-PAGE The immunoprecipitated Histone H2A.Z protein was detected by Histone H2A.Z antibody (OAGA00660) diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

Histone H2A.Z antibody immunoprecipitates Histone H2A.Z protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 ug preimmune rabbit IgG B. 5 ug of Histone H2A.Z antibody (OAGA00660) The precipitated DNA was detected by PCR with primer set targeting to GAPDH gene locus.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: CHIP, ICC, IF, IHC-FFPE, IP, WB
• Additional Information: Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq]
  Positive Controls: Molt-4, Raji
  Cellular Localization: Nucleus
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Synthetic peptide corresponding to a region within amino acids 65 and 158 of Histone H2A.Z
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000, IP: 1:500-1:1000, ChIP. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: H2AFZ
• Gene Full Name: Histone H2A.Z
• Alias Symbols: H2AZ, H2A.z, H2A/z, H2AFZ, H2A.Z-1
• NCBI Gene Id: 3015
• Protein Name: Histone H2A.Z
• Uniprot ID: P0C0S5
• Protein Accession #: NP_002097.1
• Molecular Weight: 14 kDa