Catalog No: OKEH02433
Price: $625.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

H2AFX ELISA Kit (Mouse) : 96 Wells (OKEH02433)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology H2AFX ELISA Kit (Mouse) (OKEH02433) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for H2AFX has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for H2AFX is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample H2AFX captured in well.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.3% (n = 20)
Mean Inter-assay CV%: < 7.5% (n = 20)
ELISA Kit Component
H2AFX Microplate96 Wells (12 x 8 Well strips)
H2AFX Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated GLO1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity15.61 pg/mL
SpecificityNatural and recombinant Mouse Histone H2AX
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolH2ax
Gene Full NameH2A.X variant histone
Alias SymbolsAW228881;gammaH;gammaH2ax;H2A histone family, member X;H2A.;H2A.X;H2a/x;H2af;H2afx;Hist5-;Hist5-2ax;histone 5 protein 2ax;histone H2A.x;histone H2AX.
NCBI Gene Id15270
Protein NameP27661
Description of TargetHistone H2AX
Uniprot ID
Protein Accession #NP_034566.1
Nucleotide Accession #NM_010436.2
Protein Size (# AA)143
Molecular Weight15 kDa
Write Your Own Review
You're reviewing:H2AFX ELISA Kit (Mouse) : 96 Wells (OKEH02433)
Your Rating