H1F0 ELISA Kit (Human) (OKCD08176)

Catalog No: OKCD08176

Size:96WELLS

H1F0 ELISA Kit (Human) (OKCD08176) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone H1 (H1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histone H1 (H1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histone H1 (H1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Histone H1 (H1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histone H1 (H1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histone H1 (H1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • H1F0 Microplate: 96 Wells (12 x 8 Well strips)
  • H1F0 Lyophilized Standard: 2
  • 100X H1F0 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.056ng/mL

Target Info

• Gene Symbol: H1-0
• Gene Full Name: H1.0 linker histone
• Alias Symbols: H1 histone family member 0;H1.0;H1.0, H1(0), H1-0;H10;H1F0;H1FV;histone H1';histone H1(0);histone H1.0.
• NCBI Gene Id: 3005
• Protein Name: Histone H1.0
• Description of Target: Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.
• Uniprot ID: P07305
• Protein Accession #: NP_005309.1
• Nucleotide Accession #: NM_005318.3