GZMK ELISA Kit (Mouse) (OKCD00170)

Catalog No: OKCD00170 (Formerly GWB-KBBLQ2)

Size:96WELLS

Mouse Gzmk ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	94-102%	98-105%	78-93%	84-92%
  EDTA plasma(n=5)	84-98%	90-101%	93-101%	90-101%
  heparin plasma(n=5)	80-93%	82-99%	85-92%	90-104%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Granzyme K (GZMK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Granzyme K (GZMK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Granzyme K (GZMK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Granzyme K (GZMK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	83-93	90
  EDTA plasma(n=5)	79-102	99
  heparin plasma(n=5)	81-101	87

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Granzyme K (GZMK) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Granzyme K (GZMK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Gzmk Microplate: 96 Wells (12 x 8 Well Strips)
  • Gzmk Lyophilized Standard: 2 x 1 ng
  • 100X Biotinylated Gzmk Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 6.2 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Granzyme K (GZMK). No significant cross-reactivity or interference between Granzyme K (GZMK) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Gzmk
• Gene Full Name: granzyme K
• Alias Symbols: granzyme K.
• NCBI Gene Id: 14945
• Protein Name: Granzyme K
• Description of Target: Granzyme K
• Uniprot ID: O35205
• Protein Accession #: NP_032222
• Nucleotide Accession #: NM_008196.1
• Protein Size (# AA): 263