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GZMB Antibody (OASA00733)

0.1 mg
$455.00
In Stock
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Gene Symbol:
GZMB
NCBI Gene Id:
3002
Protein Name:
Granzyme B
Swissprot Id:
P10144
Protein Accession #:
NP_004122.2
Alias Symbols:
HLP, CCPI, CGL1, CSPB, SECT, CGL-1, CSP-B, CTLA1, CTSGL1, GZMB
Replacement Item:
This antibody may replace item sc-114114 from Santa Cruz Biotechnology.
Description of Target:
MOUSE ANTI GRANZYME B
Protein Size (# AA):
247
Host:
Mouse
Clonality:
Monoclonal
Application:
IHC-AFF, ELISA, FC, IP
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express GZMB.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express GZMB.
Immunogen:
The immunogen for anti-GZMB antibody: purified human Granzyme B
Predicted Species Reactivity:
Human
Predicted Homology Based on Immunogen Sequence:
Human
Product Format:
Purified IgG - liquid
Reconstitution and Storage:
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Datasheets/Manuals:
Printable datasheet for anti-GZMB antibody - OASA00733
Specificity:
GRANZYME B
Clone:
GB11
Isotype:
IgG1
Application Info:
ELISA : This antibody has been reported to function as a capture reagent in sandwich ELISA assays for soluble Granzyme B in conjunction with biotin conjugated clone GB10 (OASA00734) as detection reagent.
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Application Data:
Application #1: Staining of peripheral blood lymphocytes with Mouse anti Human Granzyme B: RPE following permeabilization with Leucoperm (BUF09)
Additional Information:
Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
:::
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preservative Stabilisers: 0.09% - Sodium azide
:::
Approx Protein Conc: IgG concentration 1.0 mg/ml
Buffer Solutions: Tris buffered saline
Target Reference:
1. Spaeny-Dekking, E. H. et al. (1998) Extracellular Granzymes A and B in humans: Detection of native species during CTL responses in vitro and in vivo. J. Immunol. 160: 3610-3616.
2. Tschopp, C.M. (2006) Granzyme B, a novel mediator of allergic inflammation: its induction and release in blood basophils and human asthma.

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