- NCBI Gene Id:
- Protein Name:
- Granzyme B
- Alias Symbols:
- HLP, CCPI, CGL1, CSPB, SECT, CGL-1, CSP-B, CTLA1, CTSGL1, GZMB
- Replacement Item:
- This antibody may replace item sc-114114 from Santa Cruz Biotechnology.
- Description of Target:
- MOUSE ANTI GRANZYME B
- Protein Size (# AA):
- IHC-AFF, ELISA, FC, IP
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express GZMB.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express GZMB.
- The immunogen for anti-GZMB antibody: purified human Granzyme B
- Predicted Species Reactivity:
- Predicted Homology Based on Immunogen Sequence:
- Product Format:
- Purified IgG - liquid
- Reconstitution and Storage:
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Printable datasheet for anti-GZMB antibody - OASA00733
- GRANZYME B
- Application Info:
- ELISA : This antibody has been reported to function as a capture reagent in sandwich ELISA assays for soluble Granzyme B in conjunction with biotin conjugated clone GB10 (OASA00734) as detection reagent.
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
- Application Data:
- Application #1: Staining of peripheral blood lymphocytes with Mouse anti Human Granzyme B: RPE following permeabilization with Leucoperm (BUF09)
- Additional Information:
- Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preservative Stabilisers: 0.09% - Sodium azide
- Approx Protein Conc: IgG concentration 1.0 mg/ml
Buffer Solutions: Tris buffered saline
- Target Reference:
- 1. Spaeny-Dekking, E. H. et al. (1998) Extracellular Granzymes A and B in humans: Detection of native species during CTL responses in vitro and in vivo. J. Immunol. 160: 3610-3616.
2. Tschopp, C.M. (2006) Granzyme B, a novel mediator of allergic inflammation: its induction and release in blood basophils and human asthma.