GZMB Antibody (OAEE00403)

Catalog No: OAEE00403

Size:100UG

Flow cytometry analysis of human CD8+ peripheral blood cells with anti-human granzyme B (CLB-GB11) PE. 

Product Info

• Predicted Species Reactivity: Human|Non-human Primate
• Product Format: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: CLB-GB11
• Isotype: IgG1
• Host: Mouse
• Application: Flow cytometry
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: Human NK cell line YT-INDY-derived granzyme B
• Purification: Purified
• Concentration: 1 mg/ml
• Specificity: The mouse monoclonal antibody CLB-GB11 recognizes granzyme B, a 31 kDa serine protease expressed in activated Tc cells and NK cells.
• Formulation: Purified
• Purity: > 95% (by SDS-PAGE)
• Storage Buffer: Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
• Reference: Sun Y, Schmitz JE, Acierno PM, Santra S, Subbramanian RA, Barouch DH, Gorgone DA, Lifton MA, Beaudry KR, Manson K, Philippon V, Xu L, Maecker HT, Mascola JR, Panicali D, Nabel GJ, Letvin NL: Dysfunction of simian immunodeficiency virus/simian human immunodeficiencyvirus-induced IL-2 expression by central memory CD4+ T lymphocytes. J Immunol. 2005 Apr 15;174(8):4753-60.
  Rissoan MC, Duhen T, Bridon JM, Bendriss-Vermare N, Péronne C, de Saint Vis B, Brière F, Bates EE: Subtractive hybridization reveals the expression of immunoglobulin-like transcript 7, Eph-B1, granzyme B, and 3 novel transcripts in human plasmacytoid dendritic cells. Blood. 2002 Nov 1;100(9):3295-303.
  Tak PP, Spaeny-Dekking L, Kraan MC, Breedveld FC, Froelich CJ, Hack CE: The levels of soluble granzyme A and B are elevated in plasma and synovial fluid of patients with rheumatoid arthritis (RA). Clin Exp Immunol. 1999 May;116(2):366-70.
  Spaeny-Dekking EH, Hanna WL, Wolbink AM, Wever PC, Kummer JA, Swaak AJ, Middeldorp JM, Huisman HG, Froelich CJ, Hack CE: Extracellular granzymes A and B in humans: detection of native species during CTL responses in vitro and in vivo. J Immunol. 1998 Apr 1;160(7):3610-6.
  Martin P, Pardo J, Schill N, Joeckel L, Berg M, Froelich CJ, Wallich R, Simon MM: Granzyme B-induced and caspase 3-dependent cleavage of gelsolin by mouse cytotoxic T cells modifies cytoskeleton dynamics. J Biol Chem. 2010 Apr 15. [Epub ahead of print]
  Kummer JA, Kamp AM, Tadema TM, Vos W, Meijer CJ, Hack CE: Localization and identification of granzymes A and B-expressing cells in normal human lymphoid tissue and peripheral blood. Clin Exp Immunol. 1995 Apr;100(1):164-72.
  Kummer JA, Wever PC, Kamp AM, ten Berge IJ, Hack CE, Weening JJ: Expression of granzyme A and B proteins by cytotoxic lymphocytes involved in acute renal allograft rejection. Kidney Int. 1995 Jan;47(1):70-7.

Target Info

• Gene Symbol: GZMB
• Gene Full Name: granzyme B
• Alias Symbols: C11;cathepsin G-like 1;CCPI;CGL1;CGL-1;CSPB;CSP-B;CTLA1;CTLA-1;CTSGL1;cytotoxic serine protease B;cytotoxic T-lymphocyte proteinase 2;fragmentin 2;Fragmentin-2;granzyme B;granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1);Granzyme-2;HLP;human lymphocyte protein;SECT;T-cell serine protease 1-3E.
• NCBI Gene Id: 3002
• Protein Name: Granzyme B
• Description of Target: This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
• Uniprot ID: P10144
• Protein Accession #: NP_004122.2
• Nucleotide Accession #: NM_004131.4