GZMA ELISA Kit (Mouse) (OKCD02583)

Catalog No: OKCD02583

Size:96WELLS

Mouse GZMA ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	81-91%	80-88%	80-96%	81-92%
  EDTA Plasma (n=5)	81-89%	87-96%	93-101%	86-96%
  Heparin Plasma (n=5)	82-89%	78-89%	90-104%	78-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Granzyme A (GZMA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Granzyme A (GZMA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Granzyme A (GZMA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Granzyme A (GZMA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 3.12-200pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	82-92	85
  EDTA Plasma (n=5)	87-102	95
  Heparin Plasma(n=5)	85-93	88

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Granzyme A (GZMA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Granzyme A (GZMA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-GZMA Microplate: 96 Wells (12 x 8 Well strips)
  • GZMA Lyophilized Standard: 2
  • 100X Biotinylated GZMA Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 35 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Granzyme A (GZMA). No significant cross-reactivity or interference between Granzyme A (GZMA) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Gzma
• Gene Full Name: granzyme A
• Alias Symbols: autocrine thymic lymphoma granzyme-like serine protease;AW494114;BLT esterase;Ctl;Ctla;Ctla3;Ctla-3;fragmentin-1;granzyme A;H factor;Hanukah factor;Hf;Hf1;SE;SE1;serine esterase 1;t cell-specific serine protease 1;TS;TSP;TSP1;TSP-1.
• NCBI Gene Id: 14938
• Protein Name: Granzyme A
• Description of Target: Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent cell death with morphological features of apoptosis when delivered into the target cell through the immunological synapse. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA (By similarity).
• Uniprot ID: P11032
• Protein Accession #: NP_034500.1
• Nucleotide Accession #: NM_010370.2
• Protein Size (# AA): 260