GZMA Antibody (OAAF03023)

Catalog No: OAAF03023 (Formerly GWB-ASE176)

Size:100 ug

Immunohistochemistry analysis of paraffin-embedded human heart tissue, using GRAA Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HepG2 cells, using GRAA Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human GRAA.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: GRAA antibody detects endogenous levels of total GRAA protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:5000
• Reference: The MGC Project Team, e Res. 14:2121-2127(2004).
  Bell J.K., Nat. Struct. Biol. 10:527-534(2003).
  Hink-Schauer C., Nat. Struct. Biol. 10:535-540(2003).

Target Info

• Gene Symbol: GZMA
• Gene Full Name: granzyme A
• Alias Symbols: CTL tryptase;CTLA3;cytotoxic T-lymphocyte proteinase 1;Cytotoxic T-lymphocyte-associated serine esterase-3;fragmentin-1;granzyme 1;granzyme A;Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factor serine protease);granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3);Granzyme-1;h factor;Hanukah factor serine protease);Hanukkah factor;HF;HFSP.
• NCBI Gene Id: 3001
• Protein Name: Granzyme A
• Description of Target: Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent cell death with morphological features of apoptosis when delivered into the target cell through the immunological synapse. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA.
• Uniprot ID: P12544
• Protein Accession #: NP_006135.1
• Molecular Weight: 28 kDa