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Catalog No: OKCA02393
Size:96 Wells
Price: $754.00
SKU
OKCA02393
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean Recovery (%)Range (%)
Neat105103-107
1:29793-101
1:49184-97
1:89489-99
ELISA Kit PrincipleAviva Systems Biology Gucy2c ELISA Kit (Mouse) (OKCA02393) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gucy2c has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Gucy2c is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Gucy2c captured in the well.
ELISA Kit Range39 - 2,500 pg/mL
ELISA Kit Recovery
Sample TypeMean Recovery (%)Range (%)
Serum (n=5)10399-106
EDTA Plasma (n=4)9183-97
ELISA Kit ReproducibilityMean Intra-Assay: CV <12% (n=20)
Mean Inter-Assay: CV <15% (n=24)
ELISA Kit Component
ComponentAmount
Gucy2c Microplate96 Wells (12 x 8 Well strips)
Gucy2c Lyophilized Standard2
100X Biotinylated Gucy2c Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity9.77 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolGUCY2C
Alias SymbolsGcc, GC-C, AI893437
NCBI Gene Id14917
Protein NameHeat-stable enterotoxin receptor
Description of TargetReceptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptide guanylin.
Uniprot IDQ3UWA6
Protein Accession #NP_001120790
Nucleotide Accession #NM_001127318
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