GUCY2C ELISA Kit (Human) (OKEH02382)

Catalog No: OKEH02382

Size:96T

Human GUCY2C ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology GUCY2C ELISA Kit (Human) (OKEH02382) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GUCY2C has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GUCY2C is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GUCY2C captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.2% (n = 20)
  Mean Inter-assay CV%: < 7.8% (n = 20)
• ELISA Kit Component:
  • GUCY2C Microplate: 96 Wells (12 x 8 Well strips)
  • GUCY2C Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated GUCY2C Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.078 ng/mL
• Specificity: Natural and recombinant Human Heat-stable enterotoxin receptor
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: GUCY2C
• Gene Full Name: guanylate cyclase 2C
• Alias Symbols: DIAR6;GC-C;guanylyl cyclase C;GUC2C;heat-stable enterotoxin receptor;intestinal guanylate cyclase;MECIL;MUCIL;STA receptor;STAR.
• NCBI Gene Id: 2984
• Protein Name: P25092
• Description of Target: Heat-stable enterotoxin receptor
• Uniprot ID: https://www.uniprot.org/uniprot/P25092
• Protein Accession #: NP_004954.2
• Nucleotide Accession #: NM_004963.3
• Protein Size (# AA): 1073
• Molecular Weight: 123 kDa